Title:
MULTIPLEX RNA-GUIDED GENOME ENGINEERING
Document Type and Number:
Japanese Patent JP2023021447
Kind Code:
A
Abstract:
To iterate or repeat steps of DNA modification on a cell to create a cell having multiple DNA modifications in the cell.SOLUTION: Methods of multiplex genome engineering in a cell using Cas9 is provided which include a cycle of a step of introducing into the cell a first foreign nucleic acid encoding one or more RNAs which are complementary to a target DNA and which guide an enzyme to the target DNA, where the one or more RNAs and the enzyme are constituents of a co-localization complex for the target DNA, and a step of introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, where the cycle is repeated a desired number of times to perform multiplex DNA modification in the cell.SELECTED DRAWING: None
Inventors:
Church, George, Em.
DiCaro, James
DiCaro, James
Application Number:
JP2022202204A
Publication Date:
February 10, 2023
Filing Date:
December 19, 2022
Export Citation:
Assignee:
President and Fellows of Harvard College
International Classes:
C12N5/10; C12N1/19
Attorney, Agent or Firm:
Kato Kazuho
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