Title:
変異型エンドヌクレアーゼ
Document Type and Number:
Japanese Patent JP4822801
Kind Code:
B2
Abstract:
A novel mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but showing a specific activity. The present invention relates to a mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but having a specific activity, to a gene coding for the endonuclease V, to a recombinant DNA containing the gene, to a transformant or transductant containing the recombinant DNA, and to a method for producing the endonuclease V. The present invention also relates to a method of using the endonuclease V for nucleic acid cleavage, and to a reagent kit for detection or modification of a nucleic acid or gene that contains the endonuclease V.
Inventors:
Katsunori Nakajima
Oiso Isao
Oiso Isao
Application Number:
JP2005308533A
Publication Date:
November 24, 2011
Filing Date:
October 24, 2005
Export Citation:
Assignee:
Nishikawa Rubber Industry Co., Ltd.
International Classes:
C12N9/16; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12Q1/68
Other References:
Biochemistry,2002年,Vol.41,p.8342-8350
Biochemistry,2005年 8月,Vol.44,p.11486-11495
Biochemistry,2005年 8月,Vol.44,p.11486-11495
Attorney, Agent or Firm:
Shohei Oguri
Hironori Honda
Toshimitsu Ichikawa
Hironori Honda
Toshimitsu Ichikawa