Title:
MUTANT FARNESYL DIPHOSPHATE SYNTHETASE CAPABLE OF SYNTHESIZING GERANYLGERANYL DIPHOSPHATE AND DNA CODING THE ENZYME
Document Type and Number:
Japanese Patent JP3120684
Kind Code:
B
Abstract:
PURPOSE: To obtain the subject new mutant enzyme obtained by a genetic engineering technique using a mutant gene originated from Bacillus stearothermophilus and effective for the synthesis of geranylgeranyl diphosphate playing a role in a biosynthetic path.
CONSTITUTION: A new DNA containing an amino acid sequence including the amino acid sequence of the formula, etc., coding for a mutant farnesyl diphosphate synthetase effective for the synthesis of geranylgeranyl diphosphate found in a biosynthesis path of a chain isoprenoid compound playing an important role on the life activity of various organisms and useful e.g. for the genetic engineering production of the enzyme. The DNA can be produced by performing a mutation treatment of a gene coding for a farnesyl diphosphate synthetase originated from Bacillus stearothermophilus, subjecting the mutant gene to expression and selecting a gene capable of producing an enzyme to synthesize geranylgeranyl diphosphate.
More Like This:
Inventors:
Koike, Ayumi
Obata, Atsuo
Nishino, Tokuzo
Onuma, Shinichi
Nakazawa, Takeshi
Ogura, Kyozo
Furuyama, Tanetoshi
Obata, Atsuo
Nishino, Tokuzo
Onuma, Shinichi
Nakazawa, Takeshi
Ogura, Kyozo
Furuyama, Tanetoshi
Application Number:
JP1995000025253
Publication Date:
October 20, 2000
Filing Date:
February 14, 1995
Export Citation:
Assignee:
TOYOTA MOTOR CORP
International Classes:
G01N33/50; C07H21/04; C12N1/21; C12N9/10; C12N9/12; C12N15/09; C12N15/54; C12P9/00; C12P11/00; C12R1/07; C12R1/19; (IPC1-7): C12N9/12; C12N1/21; C12N15/09; C12P11/00