Title:
変異型キシラナーゼ、その製造方法及び用途、並びにリグノセルロースの糖化物製造方法
Document Type and Number:
Japanese Patent JP5889331
Kind Code:
B2
Abstract:
What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable mutant xylanase and provision of a thermostable mutant xylanase that has substitute amino acid residues, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable mutant xylanase, and a thermostable mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50 °C for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has substitute amino acid residues.
Inventors:
Hisahi Yanai
Hiroki Tamai
Masami Nagabe
Yokoyama Fumikazu
Kaoru Okakura
Atsushi Inoue
Hiroki Tamai
Masami Nagabe
Yokoyama Fumikazu
Kaoru Okakura
Atsushi Inoue
Application Number:
JP2013545974A
Publication Date:
March 22, 2016
Filing Date:
November 22, 2012
Export Citation:
Assignee:
Mitsui Chemicals, Inc.
meiji seika Pharma Co., Ltd.
meiji seika Pharma Co., Ltd.
International Classes:
C12N15/09; A21D8/04; A23K20/00; A23K20/189; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N9/24; C12P19/14; D06L4/40
Domestic Patent References:
| JP10215866A | ||||
| JP2000166485A | ||||
| JP2001017180A |
Foreign References:
| WO2011065449A1 | ||||
| WO2007115391A1 |
Other References:
Database DDBJ/EMBL/GenBank [online], Accession No. CAD33900,,14-NOV-2006 uploaded,[retrieved on 2006-06-01],ALCOCER,M.J.et al.,Definition:endo-1,4-xylanase B [Penicillium funiculosum]
Attorney, Agent or Firm:
Atsushi Nakajima
Kato Kazunori
Hiroshi Fukuda
Kato Kazunori
Hiroshi Fukuda