Title:
ＮＡＤＰＨの精製プロセス
Document Type and Number:
Japanese Patent JP6510074
Kind Code:
B2
Abstract:
The present invention relates to a process for purifying crude NADPH product prepared by biocatalysis. The objective of the present invention is to solve the technical problems of low yield and low purity of the purified product in the existing ion exchange resin method. The present invention comprises sequentially the following steps: pretreatment, loading onto an ion column, elution of cations, pre-elution of impurities etc. The yield of the purification process disclosed in the present invention can be up to 85% or higher and the purified NADPH has a purity of up to 98% or higher.
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Inventors:
Zhang Qi
Zhang Fumin
Eiho Tera
Zhang Fumin
Eiho Tera
Application Number:
JP2017559118A
Publication Date:
May 08, 2019
Filing Date:
December 14, 2016
Export Citation:
Assignee:
Hotai Biological Engineering (Shenzhen) Co., Ltd.
Kang Tai Jiangxi Green Biosynthetic Ecological Industrial Park Development Co., Ltd.
Kang Tai Jiangxi Green Biosynthetic Ecological Industrial Park Development Co., Ltd.
International Classes:
C07H21/02; B01D61/02; B01D61/14; B01D61/58; B01D63/02; B01D63/10; B01D69/00; B01J41/05; B01J41/14; B01J49/07; B01J49/57; B01J49/60; C12P19/36
Foreign References:
| CN105463043A | ||||
| CN104892710A | ||||
| CN104876993A | ||||
| CN104876994A | ||||
| CN103233051A | ||||
| US3749709 |
Other References:
Journal of Molecular Catalysis B: Enzymatic,2003年,Vol.24-25,p.39-52
Current Analytical Chemistry,2006年,Vol.2(4),p.379-388
Current Analytical Chemistry,2006年,Vol.2(4),p.379-388
Attorney, Agent or Firm:
Hiroshi Oue