RECOVERY OF PURIFIED ANIMAL GROWTH HORMONE

Japanese Patent JP2568376

B2

PURPOSE: To obtain a purified animal growth hormone in a high yield by expressing a plasmid encoding an animal growth hormone or a polypeptide having the same amino acid sequence as the hormone and subsequently subjecting the expression product to a gel filtration chromatography and ion exchange chromatography.
CONSTITUTION: This method for recovering a purified animal growth hormone comprises expressing a plasmid encoding the animal growth hormone or a polypeptide analogue having the same amino acid sequence as the animal growth hormone and having its biological activity in bacterial cells, crushing the cell walls of the cells in a buffer solution having a neutral pH, recovering the precipitated genetic product, suspending the product in distilled water, treating the precipitate-containing suspension with an aqueous sodium hydroxide solution to solubilize the precipitates, subjecting the treated product to gel filtration chromatography to separate the solubilized hormone or the polypeptide analogue from other soluble components, and subsequently subjecting the hormone or the polypeptide analogue to ion exchange chromatography, thereby recovering the purified animal growth hormone or its polypeptide analogue in a high yield.

Heim Ave Eve
Marian Goretsuki
Av Igudle Rev Anon
Amos Otzpenheim
TakeVua Fogel
Elysia Zelon
Menahem Zeevui

JP30189893A

January 08, 1997

December 01, 1993

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Bio-Technology General Corporation

A61K35/74; A61K38/27; C07H21/04; C07K14/00; C07K14/485; C07K14/52; C07K14/575; C12N15/09; C07K14/61; C12N1/20; C12N1/21; C12N9/02; C12N15/00; C12N15/18; C12N15/53; C12N15/73; C12P21/00; C12P21/02; A61K38/00; C12R1/19; C12R1/91; C12N; (IPC1-7): C12P21/02

Yoshio Kawaguchi

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