Title:
【発明の名称】ターゲット核酸増幅/検出システム
Document Type and Number:
Japanese Patent JP3020602
Kind Code:
B2
Abstract:
This invention relates to the use of functional reporter molecules in the detection and measurement of RNA sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of nucleotide sequences, one having a probe sequence linked to a sequence capable of initiating replication by an RNA-dependent RNA polymerase. The other is capable of hybridizing to a strand separated from the extension product of the first nucleotide sequence after hybridization to a specific target sequence. The extension product of the second hybridized nucleotide sequence serves as a template source for autocatalytic replication by the RNA-dependent RNA polymerase. The replication products are detected as a means for detection of nucleic acid sequences.
Inventors:
Chu, Barbara Chen Faye
Joyce, Gerald Francis
Auger, Leslie Eleather
Joyce, Gerald Francis
Auger, Leslie Eleather
Application Number:
JP50222190A
Publication Date:
March 15, 2000
Filing Date:
December 05, 1989
Export Citation:
Assignee:
The Soak Institute For Biological Studies
International Classes:
C12Q1/68; C12Q1/6867; (IPC1-7): C12Q1/68; C12N15/09
Attorney, Agent or Firm:
Akira Asamura (3 outside)