DETERMINATION OF ENDOTOXIN IN LIPOSOME DIPSERSION

Title:

DETERMINATION OF ENDOTOXIN IN LIPOSOME DIPSERSION

Document Type and Number:

Japanese Patent JPH08290

Kind Code:

A

Abstract:

PURPOSE: To provide a method for easily and accurately determining the quantity of endotoxin in a liposome dispersion.

CONSTITUTION: The quantity of endotoxin in a liposome dispersion is determined by a synthetic substrate method by adjusting the lipid concentration of the liposome dispersion to ≤4.0g/dl, measuring the absorbances Abs (treated) of the prepared specimen treated with a reaction reagent, Abs (untreated) of the specimen treated with water, Abs (blank) of the blank specimen and Abs(standard) of the standard liquid and calculating the concentration (C) of endotoxin in the liposome dispersion from the measured values by the formula, C= Et×{[Abs (treated)-Abs (untreated)-Abs (blank)]/[Abs (standard)Abs (blank)]}.

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Inventors:

ENDOU SAORI
MORISAWA KAZUYA
AKAMA KAZUHIRO
TOKUYAMA SATORU

Application Number:

JP16632694A

Publication Date:

January 09, 1996

Filing Date:

June 23, 1994

Export Citation:

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Assignee:

NIPPON OILS & FATS CO LTD

International Classes:

G01N21/79; C12Q1/37; (IPC1-7): C12Q1/37; G01N21/79

Attorney, Agent or Firm:

Ogi

Next Patent: DETERMINATION OF ALPHA-AMYLASE ACTIVITY AND REAGENT THEREFOR