Title:
ANTIGLUTATHIONE ANTIBODY AND ITS PRODUCTION
Document Type and Number:
Japanese Patent JP3025633
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To produce a new antiglutathione antibody protein, available from a certain kind of a phage display library without immunizing an animal, capable of bonding to glutathione and a protein modified with the glutathione and useful for detecting or determining the glutathione.
SOLUTION: This new antibody protein is an immunologically active polypeptide, containing at least an amino acid sequence represented by formula I to IV In a CDR3 region or its derivative in which one or more amino acids are deficient, inserted or substituted or its active fragment and capable of bonding to glutathione or a protein modified with the glutathione. The protein is useful for detecting or determining the glutathione essential for controlling a redox state of a cytoplasmic reticulum and making a protein form a correct structure. The antibody is obtained by finding a DNA capable of coding a polypeptide capable of specifically bonding to the glutathione from a certain kind of a phage display library without performing operations to immunize a mammal such as a mouse with an antigen and then expressing the resultant DNA.
Inventors:
Masakazu Kikuchi
Hiro Mika
Hiro Mika
Application Number:
JP31687295A
Publication Date:
March 27, 2000
Filing Date:
December 05, 1995
Export Citation:
Assignee:
Protein Engineering Laboratory Co., Ltd.
International Classes:
G01N33/53; A61K39/395; C07H21/04; C07K16/00; C07K16/44; C12N1/21; C12N15/09; C12P21/02; G01N33/531; C12R1/19; (IPC1-7): C12N15/09; C07K16/44; C12N1/21; C12P21/02; G01N33/53; G01N33/531
Attorney, Agent or Firm:
Aoyama Ryo (2 outside people)
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