PURPOSE: To massively and readily obtain an N-acylneuraminic acid aldolase in an ordinary medium free from sialic acid in an industrial scale by employing the N-acylneuraminic acid aldolase-producing bacterium of genus Escherichia transformed with a new recombined plasmid.
CONSTITUTION: A recombined plasmid pMK6 which has been recombined with a DNA fragment having a size of 1.2 kb and which is the Hind-III-EcoRI fragment of a chromosomal DNA containing an N-acylneuraminic acid aldolase gene originated from an N-acylneuraminic acid aldolase-producing bacterium belonging to the genus Escherichia is first introduced into the chromosomal DNA-donating bacterium to produce the transformant [e.g. Escherichia.coli K 12C600/pMK6(FERM P-833). The transformant is cultured, and the objective enzyme is then collected from the cultured product. The culture is preferably performed at 28-37°C at a pH of 6-9 for 10-50hr by a vibration-culturing method or by an aeration-stirring method.
TSUKADA YOJI
OTA YASUHIRO
KIMURA HIKARI
| JPS611384A | 1986-01-07 |