PURPOSE: To obtain an antibiotic substance useful as an antibacterial agent, etc., on an industrial scale at a low cost by treating a specific 16-membered ring macrolide antibiotic substance with a microorganism belonging to the genus Phialophora or Preussia, thereby deacylating the 3-site of the compound.
CONSTITUTION: The objective new macrolide antibiotic substance such as 3- deacylated compound expressed by formula II or formula III can be produced by adding a 16-membered ring macrolide antibiotic substance expressed by formula I (R1 is acetyl or propionyl; R2 is H; R3 is OH or acetoxy; R2 and R3 may together with bonding carbon atom form a carbonyl; R4 is H or acetyl; R5 is H, acetyl, propionyl, butyryl or isovaleryl) to a medium containing a microbial strain belonging to the genus Phialophora or Preussia [e.g. Phialophora Sp. PF1083 (FERM P-12281)] or an enzyme produced by the microorganism, carrying out the cultivation or reaction of the components, extracting the product from the cultured or reacted liquid and purifying the extract by silica gel column chromatography, etc.
GOMI SHUICHI
AJITO KEIICHI
YAGUCHI TAKASHI
TANAKA ERIKO
HARA OSAMU
MIYAJI SHINJI
