Title:
天然PrPSc特異的抗体
Document Type and Number:
Japanese Patent JP3586275
Kind Code:
B2
Abstract:
An antibody is claimed which is characterised by its ability to bind the scrapie isoform of prion protein PrP-Sc in situ. Also claimed are: (1) an antibody which specifically binds to PrP-Sc produced by a process comprising: (a) synthesizing a library of antibodies on phage; (b) panning the library against a sample by bringing the phage into contact with a compsn. comprising prion proteins (PrP); (c) isolating phage which bind PrP-Sc protein; (2) a method of detecting human PrP-Sc in a source, comprising: (a) contacting a source suspected of contg. human PrP-Sc with an antibody which specifically binds 50% or more of human PrP-Sc in the source; and (b) determining whether the antibody binds specifically to any material in the source; and (3) an assay, comprising: (a) a support surface; and (b) an antibody bound to the surface of the support, the antibody characterised by an ability to bind native PrP-Sc in situ with a binding affinity of 107 l/mole or more.
Inventors:
Pulsinar Stanley Bee.
Williamson Earl. Anthony
Burton Dennis Earl.
Williamson Earl. Anthony
Burton Dennis Earl.
Application Number:
JP2003161107A
Publication Date:
November 10, 2004
Filing Date:
June 05, 2003
Export Citation:
Assignee:
The Legends of the University of California
THE SCRIPPS RESEARCH INSTITUTE
THE SCRIPPS RESEARCH INSTITUTE
International Classes:
C07K16/18; C07K16/44; C12N5/10; G01N33/53; C12N15/02; C12N15/09; C12P21/08; G01N33/569; G01N33/68; (IPC1-7): C07K16/18; G01N33/53
Domestic Patent References:
| JP4211395A | ||||
| JP6205691A | ||||
| JP7501798A | ||||
| JP4502700A |
Other References:
Journal Cell. Biochem., Supplement 18D, Abstract T304 (1994)
Neurology, Vol.40, p.518-522 (March 1990)
The New England Journal of Medicine, Vol.314, No.9, p.547-551 (Feb.27, 1986)
The Journal of Immunology, Vol.135, No.1, p.603-613 (July 1985)
Neurology, Vol.40, p.518-522 (March 1990)
The New England Journal of Medicine, Vol.314, No.9, p.547-551 (Feb.27, 1986)
The Journal of Immunology, Vol.135, No.1, p.603-613 (July 1985)
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Kazunori Hashimoto