NERVE-SPECIFIC ENOLASE PROTEIN MANIFESTATION VECTOR AND USE OF THE SAME PROTEIN

Title:

NERVE-SPECIFIC ENOLASE PROTEIN MANIFESTATION VECTOR AND USE OF THE SAME PROTEIN

Document Type and Number:

Japanese Patent JPH06217780

Kind Code:

A

Abstract:

PURPOSE: To supply a large amount of human nerve specific enolase (NSE) by gene manipulation at a low cost.

CONSTITUTION: A DNA encoding human nerve specific enolase (NSE) is inserted into the downstream of tac promoter of manifestation vector pKK233-2 to give NSE protein manifestation vector. Further a protein obtained by manifesting the vector and use of the protein as immunogen, a labeled substance and a standard article. Since the prepared NSE protein has the same immunological properties as those of natural NSE protein, a large amount of a raw material required for immunological measurement can be inexpensively supplied.

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Inventors:

WATABE HIROYUKI
AOKI TAKASHI
MORIKAWA JUNJI

Application Number:

JP25886792A

Publication Date:

August 09, 1994

Filing Date:

September 03, 1992

Export Citation:

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Assignee:

EIKEN CHEMICAL

International Classes:

C12N9/88; C12N15/09; C12N15/60; C12P21/08; G01N33/573; C12R1/19; (IPC1-7): C12N15/60; C12N9/88; C12P21/08; G01N33/573

Attorney, Agent or Firm:

Jiro Ohara (1 outside)

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