PURPOSE: To obtain new enzymes capable of decomposing alginic acid.
CONSTITUTION: Three kinds of these enzymes are the following enzymes: (1) The enzyme cuts polyG and polyMg parts in alginic acid molecule, but does not cut polyM part and optimum pH of the reaction is in the range of 7.5-8.0 and the enzyme is stable at pH5-9. (2) The enzyme cuts every parts of polyG, polyMG and polyM parts and optimum pH of the reaction is 6.5 and the enzyme is stable at pH5-9. (3) The enzyme cuts polyG and polyMG parts, but does not cut polyM part and optimum pH of the reaction is 8.0 and the enzyme is stable at pH4-9. This invention further includes a method for purifying these enzyme and production of polymannuronic acid from alginic acid using these enzymes.
JP2009034041 | METHOD FOR MEASURING HEAVY METAL |
JP2007222187 | PROMOTERS AND USAGE THEREOF |
WO/2015/011209 | DITERPENE PRODUCTION IN YARROWIA |
KUSAKABE ISAO
MURATA KATSUMI
KIBUN SHOKUHIN KK