NEW ALKALINE PECTIC ACID LYASE AND ITS PRODUCTION

Japanese Patent JP3917258

B2

PROBLEM TO BE SOLVED: To obtain the subject new enzyme for e.g. cleaning composition, consisting of alkaline pectic lyase which scissors a α-1, 4 bond of pectic acid by β-elimination reaction and produces e.g. digalacturonide having a double bond at its unreduced terminal.
SOLUTION: This new alkaline pectic acid lyase is to has such a function as to endo-fashion scissor α-1, 4 bond of pectic acid (polygalacturonic acid) by β-elimination reaction and provide an unreduced C4-C5 site with a double bond to form unsaturated digalacturonide or unsaturated oligogalacturonide, and has a molecular weight of about 20 to 21 kDa (ultracentrifugal equilibrium sedimentation method) in a pH of 5 to 11.5 (for 60 minutes in a 40 mM Britton- Robinson universal buffer solution at 30°C), wherein the optimum pH of reaction is 10.3 to 10.7 (in a 50 mM glycine-sodium hydroxide buffer solution, using pectin as a substrate). This enzyme is useful for a cleaning composition for clothes and dishes, and can be obtained from Bacillus sp. KSM-P15 (FERM P-15987).

Kenzo Koike
Yoshimatsu Tadashi
Norihiko Higaki
Atsushi Rinmatsu
Toru Kobayashi
Susumu Ito

JP24273497A

May 23, 2007

September 08, 1997

Click for automatic bibliography generation  

Kao Corporation

C12N9/88; C07H21/04; C11D3/386; C11D7/42; C12N1/21; C12N15/00; C12N15/09; C12N15/60; C12S11/00; C11D3/39; C12R1/07; C12R1/19; (IPC1-7): C12N9/88; //C11D3/386; C11D7/42; (C12N9/88; C12R1:07)

JP10337187A

Arch. Biochem. Biophy.,1995年,Vol. 323(2),p. 352-360
Arch. Biochem. Biophy.,1987年,Vol. 258(1),p. 196-205
Can. J. Microbiol.,1980年,Vol. 26(3),p. 377-384
J. Bacteriol.,1987年,Vol. 169(6),p. 2774-2780

Alga Patent Office, a patent business corporation
Miyuki Ariga
Toshio Takano
Toshio Nakajima
Hiromi Matoba

Previous Patent: HYDRAULIC CIRCUIT DEVICE OF HYDRAULIC WORKING MACHINE

Next Patent: METHOD FOR CONNECTING STRIPS OF FILM AND DEVICE THEREFOR