NEW BACTERIUM AND PRODUCTION OF L-AMINO ACID USING THE SAME

Title:

NEW BACTERIUM AND PRODUCTION OF L-AMINO ACID USING THE SAME

Document Type and Number:

Japanese Patent JP2000270882

Kind Code:

A

Abstract:

To obtain a new bacterium useful for producing an L-amino acid, containing a plasmid containing a phenylalanine transaminase gene derived from a bacterium belonging to the genus Paracoccus at the downstream of a promoter functioning in a host bacterium.

This bacterium is obtained by adding a recombinant plasmid incorporated with a DNA encoding a phenylalanine transaminase derived from a bacterium (e.g. Paracoccus denitrificans, etc.), belonging to the genus Paracoccus at the downstream of a promoter functioning in a host bacterium to the host bacterium being Escherichia coli. The new recombinant bacterium has a base sequence between the promoter and an initiation codon of phenylalanine transaminase, being sequence of the formula. An L-amino acid can be efficiently obtained by treating the bacterium or its treated material with an oxo-acid compound in the presence of an amino acid donor.

Inventors:

MATSUMAE HIROAKI
TANIGUCHI TOMOYASU
SHIBATANI TAKEJI
YOSHIOKA RYUZO

Application Number:

JP2000011171A

Publication Date:

October 03, 2000

Filing Date:

January 20, 2000

Export Citation:

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Assignee:

TANABE SEIYAKU CO

International Classes:

C12N15/09; C12N1/21; C12N9/10; C12P13/04; C12R1/19; (IPC1-7): C12N15/09; C12N1/21; C12N9/10; C12P13/04

Other References:

JPN6009061573, Ｊ．　Ｂｉｏｃｈｅｍ．, 1997, ｖｏｌ．１２１, ｐｐ．１６１−１７１
JPN6009061575, Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ａｐｐｌｉｅｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ, 1991, ｖｏｌ．１３, ｐｐ．１１２−１１９

Attorney, Agent or Firm:

Shigeo Minoura

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