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Title:
NEW COMPOUND, METHOD FOR PRODUCING NEW COMPOUND BY UTILIZING NEW BIOSYNTHETIC ROUTE OF MENAQUINONE, METHOD FOR INCREASING QUANTITY OF PRODUCTION OF MENAQUINONE BY UTILIZING INTERMEDIATE IN NEW BIOSYNTHETIC ROUTE OF MENAQUINONE, AND METHOD FOR SEARCHING INHIBITOR SPECIFIC TO NEW BIOSYNTHETIC ROUTE OF MENAQUINONE BY UTILIZING INTERMEDIATE IN NEW SYNTHETIC ROUTE OF MENAQUINONE
Document Type and Number:
Japanese Patent JP2009114124
Kind Code:
A
Abstract:

To provide a new compound constituting a new biosynthetic route of menaquinone, a method for producing the new compound by utilizing the route, a method for increasing the quantity of production of the menaquinone by utilizing the compound, and a method for searching an inhibitor specific to the route.

A specific compound is provided. The method for producing the compound includes reacting futalosine with an enzyme for carrying out de-hypoxanthine of the futalosine or a recombinant enzyme having activity for carrying out the de-hypoxanthine of the futalosine, and a reducing agent in the presence of water or a buffer solution. The method for producing the new compound includes making an orthologous gene-broken strain of a microorganism having a new synthetic route of the menaquinone, and culturing the broken strain in the presence of the menaquinone. A method for searching an inhibitor by synthesizing a structural analogue of the compound, adding the material to a microorganism having the new biosynthetic route of the menaquinone, and identifying the structural analogue as the inhibitor specific to the new biosynthetic route of the menaquinone from proliferation-inhibiting activity of the microorganisms and inhibitory activity against the enzyme carrying out the de-hypoxanthine of the futalosine.


Inventors:
Otoshi, Toru
Seto, Haruo
Furuhata, Kazuo
Yamashita, Haruyuki
Application Number:
JP2007000289063
Publication Date:
May 28, 2009
Filing Date:
November 06, 2007
Export Citation:
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Assignee:
ADEKA CORP
International Classes:
C07H7/04; C12Q1/02; C12N15/09; C12P7/42; C12R1/01; C12R1/465; C07H7/00; C12Q1/02; C12N15/09; C12P7/40