Title:
NEW CREATININE AMIDOHYDROLASE
Document Type and Number:
Japanese Patent JP3829950
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a higher-purity creatinine amidohydrolase as compared with a conventional enzyme.
SOLUTION: This creatinine amidohydrolase is obtained from Alcaligenes faecalis TE3581 (FERM P-14237) and has the following physicochemical properties: A gene is capable of coding the enzyme and a recombinant vector contains the gene. Furthermore, a transformant is transformed with the recombinant vector. The creatinine amidohydrolase is obtained by culturing the transformant in a culture medium, producing the creatinine amidohydrolase and collecting the produced creatinine amidohydrolase. optimum temperature: 60°C; optimum pH: 7.5-8.5; thermal stability: about ≤50°C (at pH7.5 for 30min); pH stability: about 8.0-10.0 (at 4°C for 16hr); molecular weight: about 31,500 (SDS-PAGE) and about 120,000 (gel filtration) and isoelectric point: about 4.8.
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Inventors:
Yoshiya Nishiya
Atsushi Toda
Yoshihisa Kawamura
Atsushi Toda
Yoshihisa Kawamura
Application Number:
JP31429595A
Publication Date:
October 04, 2006
Filing Date:
December 01, 1995
Export Citation:
Assignee:
TOYO BOSEKI KABUSHIKI KAISHA
International Classes:
C12N9/86; C12N15/09; C07H21/04; C12N1/21; C12R1/05; C12R1/19; (IPC1-7): C12N9/86; C07H21/04; C12N1/21; C12N15/09; //(C12N9/86; C12R1:19); (C12N1/21; C12R1:19); (C12N15/09; C12R1:05)
Domestic Patent References:
| JP3019690A |
Other References:
INOUE Y;MATSUDA Y;NAID T;ARAI S;HASHIMOTO Y;ASANO K;OZAKI M;NAKAMURA S,Purification and Characterization of Creatine Amidohydrolase of Alcaligenes Origin,Chemical and Pharmaceutical Bulletin,日本,1986年,34/1,269-274
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