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Title:
NEW CROSSING METHOD FOR FUNGI
Document Type and Number:
Japanese Patent JPH07227164
Kind Code:
A
Abstract:

PURPOSE: To provide a new crossing method for fungi hitherto considered to be difficult of crossing.

CONSTITUTION: (1) A crossing method for fungi consists of selecting a primary hypha by an isozyme analysis after an isolated culturing of a protoplast of fungi having following characteristics, and selecting again the crossing fungi strain by the isozyme analysis after their dual culture. i) A nucleus of cell is polykaryotic. ii) lacking in clamp connection. iii) hard to obtain a spore. (2) A method of crossing described in (1), where the fungus is Poria and isozymes are an alcohol dehydrogenase or a fluorescent esterase. (3) A method of crossing described in (2) where a sclortium obtained has a reddish white color. By using this crossing method it becomes possible to cross fungi having characteristics having an polykaryotic nucleus of cell, lacking in the clamp connection and hardly obtaining spores, like Poria.


Inventors:
IGARI NAOKI
MINOURA NAOSUKE
OKADA MINORU
Application Number:
JP1994000043269
Publication Date:
August 29, 1995
Filing Date:
February 18, 1994
Export Citation:
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Assignee:
TSUMURA & CO
International Classes:
A01G1/04; A01H1/02; C12N1/14; (IPC1-7): A01H1/02; A01G1/04; C12N1/14