Title:
NEW ENZYME, PRODUCTION THEREOF AND PRODUCTION OF OPTICALLY ACTIVE (R)-2-HYDROXY-4-PHENYLBUTYRIC ACID
Document Type and Number:
Japanese Patent JPH04335886
Kind Code:
A
Abstract:
PURPOSE: To efficiently produce the subject optically active (R)-2-hydroxy-4- phenylbutyric acid from 2-oxo-4-phenylbutyric acid.
CONSTITUTION: An enzyme obtained by extraction and purification from a microorganism belonging to Leuconostic genus is allowed to act on 2-oxo-4- phenylbutyric acid on the presence of reduced type nicotinamde adenine dinucleotide phosphate NADPH as a coenzyme to asymmetrically reduce it. The above-mentioned enzyme has following physicochemical properties; Optimum pH: about 6.0, Stable pH region: 6-7, Optimum temperature: about 45°C. Thermal stability: ≤40°C, Michaelis constant value Km for 2-oxo-4-phenylbutyric acid: 0.29mM, Molecular weight: 46000 and Inhibitor: mercury chloride and mercury para-chlorobenzoate.
Inventors:
SODA KENJI
NAKAJIMA NOBUYOSHI
NAKAJIMA NOBUYOSHI
Application Number:
JP13196491A
Publication Date:
November 24, 1992
Filing Date:
May 07, 1991
Export Citation:
Assignee:
DAICEL CHEM
International Classes:
C12N9/04; C12P7/52; C12P41/00; C12R1/01; (IPC1-7): C12N9/04; C12P7/52; C12P41/00
Attorney, Agent or Firm:
Mitsuo Hokita