Title:
NEW GENE, VECTOR, TRANSFORMANT USING IT AND ITS USE
Document Type and Number:
Japanese Patent JP3759794
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a DNA useful for preparing a trasnformant that is able to increase saccharose content of glucoamylase capable of being produced an large amount by solid culture and enhance stability to heat and protease.
SOLUTION: This DNA has a base sequence that is shown by the formula and contains a promoter of glucoamylase genes isolated from Aspergilus orggae and/or a protein code domain. The transformant A (FERM P-15826) of this DNA is obtained by cloning cDNA that is considered to code glucoamylase expressed highly by solid culture and chromosome DNA fragments, coupling the former with an expression vector, transfecting Saccharomyces cerevisiae to obtain a transformant B (FERM P-15827), and transfacting the latter into an Aspergillus orggae host vector system.
More Like This:
Inventors:
Yoji Hata
Hiroki Ishida
Eiji Ichikawa
Akitsugu Kawato
Koji Suginami
Satoshi Imayasu
Hiroki Ishida
Eiji Ichikawa
Akitsugu Kawato
Koji Suginami
Satoshi Imayasu
Application Number:
JP26770196A
Publication Date:
March 29, 2006
Filing Date:
September 19, 1996
Export Citation:
Assignee:
Laurel wreath
International Classes:
C12G3/02; C12N15/09; C07H21/04; C07K14/37; C07K14/41; C07K14/415; C12N1/15; C12N1/19; C12N9/34; C12N15/00; C12P7/06; C12R1/69; C12R1/865; (IPC1-7): C12N15/09; C07H21/04; C07K14/415; C12G3/02; C12N1/15; C12N1/19; C12N9/34; C12P7/06; //(C12N15/09; C12R1:69); (C12N1/15; C12R1:69); (C12N1/19; C12R1:865); (C12N9/34; C12R1:69); (C12N9/34; C12R1:865); (C12P7/06; C12R1:69); (C12P7/06; C12R1:865)
Other References:
石田博樹, 他,麹菌(A.oryzae)の個体培養で発現する新規グルコアミラーゼ遺伝子のクローニング 1,日本生物工学大会講演要旨集,1996年 9月 5日,p.137
Attorney, Agent or Firm:
Matsumoto Kuki
Toda parent man
Toda parent man