Title:
New highly efficient enzyme which changed the substrate specificity of human beta-hexosaminidase B, and gave protease resistance
Document Type and Number:
Japanese Patent JP6230158
Kind Code:
B2
Abstract:
Provided is a modified ²-subunit of human ²-hexosaminidase which has the activity derived from the ±-subunit of wild-type human ²-hexosaminidase and has the resistance to protease. A protein comprising an amino acid sequence having substitutions of the 312th to the 318th amino acids with glycine, serine, glutamic acid, proline, serine, glycine and threonine in order, respectively, in an amino acid sequence of a ²-subunit of wild-type human ²-hexosaminidase.
Inventors:
Takashi Ito
Sakuraba Hitoshi
Daisuke Tsuji
Sakuraba Hitoshi
Daisuke Tsuji
Application Number:
JP2014542171A
Publication Date:
November 15, 2017
Filing Date:
October 17, 2013
Export Citation:
Assignee:
Tokushima University
Meiji Pharmaceutical University
Intellectual Property Strategy Network Co., Ltd.
Meiji Pharmaceutical University
Intellectual Property Strategy Network Co., Ltd.
International Classes:
C12N15/09; A61K38/46; A61K48/00; A61P25/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88
Domestic Patent References:
| JP2012522809A |
Foreign References:
| WO2010082622A1 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Nobuto Oki
Satoshi Fujita
Nobuto Oki