Title:
新規タンパク質、それをコードする遺伝子及びそれらの利用法
Document Type and Number:
Japanese Patent JP4516258
Kind Code:
B2
Abstract:
The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such as Pyricularia oryzae and Rhizoctonia solani causative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract of Lyophyllum shimeji precipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least against Rhizoctonia solani or Pyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.
Inventors:
Takakura Yumi
Shigeru Kuwata
Yasuhiro Inoue
Shigeru Kuwata
Yasuhiro Inoue
Application Number:
JP2001525230A
Publication Date:
August 04, 2010
Filing Date:
September 20, 2000
Export Citation:
Assignee:
Japan Tobacco Inc.
Agriculture, Forestry and Fisheries Advanced Technology Industry Promotion Center
Agriculture, Forestry and Fisheries Advanced Technology Industry Promotion Center
International Classes:
C07K14/375; A01N37/46; A01N63/50; A01N65/00; C12N1/21; C12N15/09; C12N15/31; C12P21/02; C12Q1/68
Domestic Patent References:
| JPH08205861A | 1996-08-13 | |||
| JPH08205861A | 1996-08-13 |
Other References:
JPN6010003521, Ｊ．Ｂｉｏｔｅｃｈｎｏｌ．，Ｖｏｌ．５２（１９９６）ｐ．１１−２０
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Reiko Izumiya
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Reiko Izumiya