PURPOSE: To provide a new steroidal compound synthesized from a diosgenin compound, etc., through epoxidization, reduction, halogenation, alkylation, etc., exhibiting strong cytotoxic action on L1210 leukemia cell and useful as a carcinostatic agent.

CONSTITUTION: The objective new steroidal compound of formula II (R1 is H, OH or monosaccharide, etc., bonded through 0; R2 is H or OH; R3 is H, monosaccharide, oligosaccharide, etc.; R4 and R5 are H, OH, halogen or together form epoxy, etc.; R6 is H or form CO together with 12-C atom; X is 0 or secondary amine) can be produced by subjecting a diosgenin compound of formula I (Me is methyl) to epoxidization, alkylation, acylation, hydroxylation, glycosidation, etc., used in conventional chemical reactions.