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Title:
NEW TISSUE PLASMINOGEN ACTIVATING FACTOR
Document Type and Number:
Japanese Patent JP2606620
Kind Code:
B2
Abstract:

PURPOSE: To obtain a DNA useful for creating a plasminogen activating factor having potent activity to convert plasminogen into plasmin and useful as a thrombolytic agent.
CONSTITUTION: This DNA codes for an amino acid sequence of the formula (R is Ser, etc.; X is Lys, Ile, etc.; Y is Tyr-Ser-Gln-Pro-Gln-Phe-Arg-Ile, etc.; except that R is Ser, X is Lys, and Y is Tyr-Ser-Gin-Pro-Gin-Phe-Asp-Ile). The objective plasminogen activating factor is obtained by treating by conventional, means a complete DNA sequence or genome coding for natural or mutant t-PA transfected into a vector (e.g. pTPA21, pTPA25) obtained by partial or full DNA synthesis using a DNA synthesizer or from a transformant [e.g. E. coli LE392λ+ (pTPA21), E. coli JA221 (pTPA25) ATCC 39808]. The t-PA is active, well-reholdable, long in half-life and also excellent in thrombolytic activity.


Inventors:
Niwa, Mineo
Saito, Yoshimasa
Sasaki, Hitoshi
Hayashi, Masako
Notani, Joji
Kobayashi, Masakazu
Application Number:
JP1996000003853
Publication Date:
May 07, 1997
Filing Date:
January 12, 1996
Export Citation:
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Assignee:
FUJISAWA PHARMACEUT CO LTD
International Classes:
C12N15/09; A61K38/46; A61K38/48; A61P7/02; C07H21/04; C07K14/745; C12N1/20; C12N1/21; C12N5/10; C12N9/64; C12N9/72; C12N15/58; C12R1/19; C12R1/91; (IPC1-7): C12N1/21
Foreign References:
Other References:
PROC.NATL.ACAD.SCI=1986US