Title:
NEW TISSUE PLASMINOGEN ACTIVATING FACTOR
Document Type and Number:
Japanese Patent JP2606620
Kind Code:
B2
Abstract:
PURPOSE: To obtain a DNA useful for creating a plasminogen activating factor having potent activity to convert plasminogen into plasmin and useful as a thrombolytic agent.
CONSTITUTION: This DNA codes for an amino acid sequence of the formula (R is Ser, etc.; X is Lys, Ile, etc.; Y is Tyr-Ser-Gln-Pro-Gln-Phe-Arg-Ile, etc.; except that R is Ser, X is Lys, and Y is Tyr-Ser-Gin-Pro-Gin-Phe-Asp-Ile). The objective plasminogen activating factor is obtained by treating by conventional, means a complete DNA sequence or genome coding for natural or mutant t-PA transfected into a vector (e.g. pTPA21, pTPA25) obtained by partial or full DNA synthesis using a DNA synthesizer or from a transformant [e.g. E. coli LE392λ+ (pTPA21), E. coli JA221 (pTPA25) ATCC 39808]. The t-PA is active, well-reholdable, long in half-life and also excellent in thrombolytic activity.
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Inventors:
Niwa Mineo
Yoshimasa Saito
Sasaki Hitoshi
Hayashi Masako
Joji Noya
Masakazu Kobayashi
Yoshimasa Saito
Sasaki Hitoshi
Hayashi Masako
Joji Noya
Masakazu Kobayashi
Application Number:
JP385396A
Publication Date:
May 07, 1997
Filing Date:
January 12, 1996
Export Citation:
Assignee:
Fujisawa Pharmaceutical Co., Ltd.
International Classes:
A61K38/46; A61K38/48; A61P7/02; C07H21/04; C07K14/745; C12N1/20; C12N1/21; C12N5/10; C12N9/64; C12N15/09; C12N9/72; C12N15/58; C12R1/19; C12R1/91; (IPC1-7): C12N1/21
Other References:
PROC.NATL.ACAD.SCI=1986US