Title:
NON-ENZYMATIC IMMUNOLOGICAL TISSUE CHEMICAL COLORING SYSTEM AND REAGENT
Document Type and Number:
Japanese Patent JPS6296858
Kind Code:
A
Abstract:
A non-enzymatic method of immunohistochemical staining uses a bound electron transfer agent to achieve amplification of antibody binding to specific antigens of interest in a histology or cytology specimen. A preferred embodiment uses a bound phenazine, which is reduced with a soluble reducing agent, and which in turn reduces a tetrazolium salt, to precipitate a formazan dye at the site of primary antibody binding to the antigen of interest. Reagents and kits for use in the method are also provided.
Inventors:
SHOCHAT DAN (US)
Application Number:
JP19155286A
Publication Date:
May 06, 1987
Filing Date:
August 14, 1986
Export Citation:
Assignee:
IMMUNOMEDICS INC (US)
International Classes:
G01N33/53; G01N33/532; G01N33/543; G01N33/563; G01N33/567; G01N33/574; G01N33/58; (IPC1-7): G01N33/532; G01N33/543
Domestic Patent References:
| JPS55116259A | 1980-09-06 |
Foreign References:
| US4160645A | 1979-07-10 | |||
| US4435509A | 1984-03-06 |
Attorney, Agent or Firm:
Akaoka Mio
Previous Patent: The silicone resin constituent containing the poly siloxane copolymer
Next Patent: ABSORBER OF HETEROPHILE ANTIBODY FOR RBC OF SHEEP
Next Patent: ABSORBER OF HETEROPHILE ANTIBODY FOR RBC OF SHEEP