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Title:
NONREDUCING TERMINAL-MODIFIED MALTOOLIGOSACCHARIDE DERIVATIVE AND FRACTIONAL DETERMINATION OF ALPHA-AMYLASE ISOZYME ACTIVITY USING THE SAME
Document Type and Number:
Japanese Patent JPH059196
Kind Code:
A
Abstract:

PURPOSE: To provide the subject new compound extremely useful as a substrate for the fractional determination of α-amylase isozyme in a specimen and enabling the fractional determination of (α-amylase isozyme with a simple process in high accuracy.

CONSTITUTION: The nonreducing terminal-modified maltooligosaccharide of formula I ((n) is 2-6; R is H, aromatic chromophore, etc.; X is arylsulfonyl, arylimido, etc.; Y is H or hydrocarbon group), e.g. 2-chloro-4-nitrophenyl 65-O-(t- butyldimethyl)silyl-β--Dmaltopentaoside. The compound can be produced e.g. by reacting a D-maltooligoside of formula II with a carbonyl compound of formula III (R16 is H, methoxy, etc.; R17 is methoxy or ethoxy), subjecting the obtained compound of formula IV to dealkoxymethylidene reaction, converting the 6-hydroxyl group of the resultant partially acylated maltooligosaccharide derivative to trialkylsilyl group and deacylating the resultant compound of formula V (R18 is acyl; R19 and R20 are alkyl).


Inventors:
TOKUTAKE SHOICHI
UCHIDA RIICHIRO
KOTANI KAZUO
SAITO KAZUNORI
TOBE KOUICHIROU
Application Number:
JP1991000184028
Publication Date:
January 19, 1993
Filing Date:
June 28, 1991
Export Citation:
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Assignee:
KIKKOMAN CORP
DAIICHI PURE CHEMICALS CO LTD
SEISHIN SEIYAKU KK
International Classes:
C07H3/06; C07H15/203; C07H15/26; C12Q1/40; (IPC1-7): C07H3/06; C07H15/203; C07H15/26; C12Q1/40
Attorney, Agent or Firm:
阿形 明 (外1名)



 
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