NEW MATERIAL:A terephthalaldehyde bis(9'-acridinyl)hydrazone derivative expressed by formula I (R1 and R2 are H, lower alkyl, lower alkoxy or substituted amino group except when both R1 and R2 are H at the same time).
USE: A staining agent for differential staining technique of human chromosome having a high molecular extinction coefficient and fluorescent intensity.
PREPARATION: For example, o-chlorobenzoic acid expressed by formula II is reacted with an aniline derivative expressed by formula III to give an N- phenylanthranilic acid derivative expressed by formula IV, which is then subjected to cyclocondensation and chlorine substitution reaction with phosphorus oxychloride to afford a compound expressed by formula V. The resultant compound expressed by formula V is then reacted with hydrazine to give a 9- hydrazinoacridine derivative expressed by formula VI, which is then reacted with terephthalaldehyde to afford the aimed compound expressed by formula I.
URANO FUMIYOSHI
MARUHASHI KAZUO
