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Title:
NOVEL ENZYME AND PRODUCTION OF PROTEIN GELATINIZED PRODUCT USING SAID ENZYME
Document Type and Number:
Japanese Patent JPS6427471
Kind Code:
A
Abstract:

PURPOSE: To produce a protein gelatinized product, by reacting a transglutaminase capable of catalyzing an acylation arrangement reaction of γ-carboxylamide group of a glutamine residue in peptide chain in the absence of Ca2+ with a protein.

CONSTITUTION: A protein-containing solution or slurry having ≥1.0wt.% protein concentration is prepared. A transglutaminase capable of catalyzing an acyl arrangement reaction of γ-carboxylamide group of glutamine residue in a peptide chain in the absence of Ca2+ is added to the protein-containing solution or slurry and reacted with the protein to provide the protein gelatinized product. The above-mentioned transglutaminase is preferably added to the protein- containing solution or slurry at an amount of 0.01W2,000 unit based on 1.0g protein. Furthermore, the microorganism having transglutaminase producing- ability incudes bacterium belonging to the genus Streptoverticillium.


Inventors:
MOTOKI MASAO
OKIYAMA ATSUSHI
NONAKA MASAHIKO
TANAKA HARUO
UCHIO RYOSUKE
MATSUURA AKIRA
ANDO HIROYASU
UMEDA KOICHI
Application Number:
JP16506787A
Publication Date:
January 30, 1989
Filing Date:
July 01, 1987
Export Citation:
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Assignee:
AJINOMOTO KK
AMANO PHARMA CO LTD
International Classes:
A23C9/12; A23C19/032; A23C20/02; A23J3/00; A23L7/104; A23L9/20; A23L13/40; A23L17/00; A23L29/00; A23L29/20; C12N9/10; C12P21/00; C12R1/01; (IPC1-7): A23C19/032; A23J3/00; A23L1/03; A23L1/04; C12N9/10; C12P21/00; C12R1/01
Attorney, Agent or Firm:
Yoshio Kawaguchi (3 outside)