NOVEL MICROORGANISM, AND PRODUCTION OF BIOTIN BY FERMENTATION USING SAME

Title:

NOVEL MICROORGANISM, AND PRODUCTION OF BIOTIN BY FERMENTATION USING SAME

Document Type and Number:

Japanese Patent JPS61202686

Kind Code:

A

Abstract:

PURPOSE: To obtain a microbial strain capable of producing biotin, by integrating a DNA carrying the gene information of an enzyme participating in the biosynthesis of biotin in a vector DNA, and introducing the recombinant DNA into a specific mutant.

CONSTITUTION: A deoxyribonucleic acid (DNA) carrying the genetic information of pimelyl CoA synthetase, 7-keto-8-aminopelargonic acid aminotransferase, desthiobiotin synthetase and an enzyme participating in the conversion from desthiobiotin to biotin is obtained from Escherichia coli having released feedback inhibition by biotin, and the DNA is integrated in a vector DNA. The recombinant DNA is introduced into Escherichia coli and the obtained microbial strain is cultured to produce and accumulate biotin in the medium.

Inventors:

IFUKU OUJI
HAJI SHINICHIRO
KISHIMOTO JIRO
SAKAMOTO TETSUO
YANAGI MITSUO

Application Number:

JP4292885A

Publication Date:

September 08, 1986

Filing Date:

March 05, 1985

Export Citation:

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Assignee:

SHISEIDO CO LTD

International Classes:

C12N15/09; C12N1/20; C12N1/21; C12N15/00; C12N15/52; C12P17/18; C12R1/19; (IPC1-7): C12N1/20; C12N15/00; C12P17/18; C12R1/19

Domestic Patent References:

JPS61149091A

1986-07-07

Attorney, Agent or Firm:

Shoichi Ui (4 others)

Previous Patent: JPS61202685

Next Patent: NOVEL MALTOTETRAOSE-PRODUCING ENZYME AND PRODUCTION THEREOF