Title:
NOVEL PLASMID
Document Type and Number:
Japanese Patent JPS5832895
Kind Code:
A
Abstract:
PURPOSE: A novel plasmid that is used as a vector plasmid to introduce an additional exogenote into cells of Escherichia coli into which an exogenote has been introduced by use of a colicin E1 vector.
CONSTITUTION: A plasmid that can have coexistence in the same host cells with a colicin E1 plasmid such as pBR322 or a recombinant DNA prepared by use of the same plasmid. For example, pGA plasmid is segregated with restriction enzyme BamHI to obtain a reaction mixture containing a couple of DNA fractions with 2.8×103 base pair and 5.1×103 base pairss respectively and the mixture is treated with T4 ligase to effect the binding reaction to prepare the objective pGB1 plasmid of a cyclic DNA with 2.8×103 base pair.
Inventors:
YAGISAWA MORIMASA
MIZUKAMI TAMIO
OKA TETSUO
FURUYA AKIRA
MIZUKAMI TAMIO
OKA TETSUO
FURUYA AKIRA
Application Number:
JP12936981A
Publication Date:
February 25, 1983
Filing Date:
August 20, 1981
Export Citation:
Assignee:
KYOWA HAKKO KOGYO KK
International Classes:
C12N15/09; C07H21/04; C12N15/70; (IPC1-7): C07H21/04; C12N15/00; C12R1/19
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