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Title:
NUCLEIC ACID ADSORBENT
Document Type and Number:
Japanese Patent JP3689897
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To obtain a nucleic acid adsorbent, comprising a copolymer capable of selectively adsorbing and separating a nucleic acid from a system comprising an enzyme coexisting therein and responsive to temperature by copolymerizing monomers containing a nitrogen-containing basic monomer and N- isopropylacrylamide.
SOLUTION: This nucleic acid adsorbent comprises a copolymer prepared by copolymerizing monomers containing 2-60wt.% nitrogen-containing basic monomer and 40-98wt.% N-isopropylacrylamide. The copolymerization of the monomers containing 2-60wt.% nitrogen-containing basic monomer (e.g. N,N- dimethylaminoethyl acrylate) and 40-98wt.% N-isopropylacrylamide is carried out in a solvent such as methanol at 70°C for 5hr by adding azobisisobutyronitrile, etc., as a polymerization initiator thereto and a 0.5wt.% aqueous solution of sulfuric acid is then added to remove the methanol and residual monomers. The pH is subsequently regulated to 6.3 to thereby afford the objective nucleic acid adsorbent, having temperature responsiveness for transparently dissolving at ≤30°C and insolubilizing and depositing at ≥35°C and capable of selectively and readily separating the nucleic acid from a system containing an enzyme coexisting therein.


Inventors:
Masayuki Hattori
Ms. Kana
Kenya Makino
Application Number:
JP31546495A
Publication Date:
August 31, 2005
Filing Date:
December 04, 1995
Export Citation:
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Assignee:
JSR CORPORATION
International Classes:
C12N9/00; B01J20/26; C07H1/06; C07H21/00; C08F20/34; C08F20/52; C08F26/00; C08F220/34; C08F220/54; C08F220/60; C08F226/00; C12N15/09; C12Q1/68; (IPC1-7): C07H1/06; B01J20/26; C07H21/00
Domestic Patent References:
JP4007385A
JP5170655A
JP6254394A
JP5009229A
Foreign References:
US3681079
Attorney, Agent or Firm:
Masataka Oshima