Title:
核酸分析方法
Document Type and Number:
Japanese Patent JP5037343
Kind Code:
B2
Abstract:
This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support.
Inventors:
Fuller, Carl W
Nelson, John Earl
Nelson, John Earl
Application Number:
JP2007527745A
Publication Date:
September 26, 2012
Filing Date:
June 09, 2005
Export Citation:
Assignee:
GE Healthcare Bioscience Corporation
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
| JP2004523243A |
Other References:
RAMANATHAN, S. et al.,"Incoming nucleotide binds to Klenow ternary complex leading to stable physical sequestration of preceding dNTP on DNA.",NUCLEIC ACIDS RES.,2001年 5月15日,Vol.29, No.10,P.2097-2105
DOUBLIE, S. et al.,"An open and closed case for all polymerases.",STRUCTURE,1999年 2月15日,Vol.7, No.2,P.R31-R35
DOUBLIE, S. et al.,"An open and closed case for all polymerases.",STRUCTURE,1999年 2月15日,Vol.7, No.2,P.R31-R35
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Toshihisa Kurokawa
Hirokazu Ogura
Toshihisa Kurokawa