Title:
核酸の検出方法
Document Type and Number:
Japanese Patent JP7434742
Kind Code:
B2
Abstract:
The present invention relates to a method for detecting an RNA virus in a specimen by reverse transcription-polymerase chain reaction (RT-PCR) and a kit for carrying out the method. More specifically, the present invention relates to a test method, characterized in that the purified water, saline or a buffer for mixing with a specimen to obtain a centrifugation supernatant as an analysis sample previously contain at least one element selected from internal control DNA, forward and reverse primers that specifically hybridize to the DNA, while RT-PCR reaction solution does not contain the above-mentioned element(s) contained in the purified water, saline, or buffer, and a kit for carrying out the method.
Inventors:
Naoko Takaoka
Masamitsu Shikata
Kenji Ninomiya
Shinichiro Kobayashi
Masamitsu Shikata
Kenji Ninomiya
Shinichiro Kobayashi
Application Number:
JP2019134717A
Publication Date:
February 21, 2024
Filing Date:
July 22, 2019
Export Citation:
Assignee:
SHIMADZU CORPORATION
International Classes:
C12Q1/686; C12Q1/6888
Domestic Patent References:
| JP2015533288A | ||||
| JP2019506849A |
Foreign References:
| WO2018198682A1 | ||||
| WO2007052765A1 | ||||
| WO2019017452A1 |
Attorney, Agent or Firm:
Patent Attorney Corporation Kyoto International Patent Office
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