Title:
鏡像体選択的アミダーゼをエンコードする核酸配列
Document Type and Number:
Japanese Patent JP2004536608
Kind Code:
A
Abstract:
The invention relates to nucleic acid sequence encoding enantioselective amides with an amino acid sequence of SEQ ID NO: 2. The invention also relates to a process for fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50 mg of Zn2+/ml of fermentation medium is fed during the fermentation. The invention also relates to a process for the preparation of enatiomerically enriched carboxylic acid and/or an enatiomerically enriched carboxylic acid amide, in which a mixture of corresponding D- and L-carboxylic acid amides is contacted with a expression product according to the invention in the presence of 0.01 mM-100 mM Zn2+, whereby one of the enantiomers of the carboxylic acid amide is enatiomerically hydrolysed to from corresponding enatiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.
Inventors:
Songke, Theodorus
Tandrel, Renate, Francisca
Correval, Cornelis, Gerrit, Nice
Assema, Van, Friso, Bernardo, Yang
Pol van der, Rudolf
Tandrel, Renate, Francisca
Correval, Cornelis, Gerrit, Nice
Assema, Van, Friso, Bernardo, Yang
Pol van der, Rudolf
Application Number:
JP2003515662A
Publication Date:
December 09, 2004
Filing Date:
July 15, 2002
Export Citation:
Assignee:
DSM IP ASSETS B.V.
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N1/38; C12N5/10; C12N9/78; C12N9/80; C12N15/10; C12N15/55; C12P13/02; C12P13/04; C12P41/00; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/78; C12P13/02
Attorney, Agent or Firm:
Mitsuo Matsui