Title:
核酸の同時増幅方法
Document Type and Number:
Japanese Patent JP4130487
Kind Code:
B2
Abstract:
A method for amplification and detection of a low copy target nucleic acid includes coamplification of a high copy target nucleic acid. After a number of conventional amplification cycles which include a denaturation step, several cycles are carried out during which the denatured products are renatured for a brief period of time. This intermediate step in later cycles of the amplification process reduces the effective concentration of the high copy target nucleic acid available for amplification in later cycles, thereby making more DNA polymerase available for amplification of the low copy target nucleic acid.
Inventors:
John W. Bacchus
John W. H. Sutherland
John W. H. Sutherland
Application Number:
JP15485995A
Publication Date:
August 06, 2008
Filing Date:
June 21, 1995
Export Citation:
Assignee:
Ortho-Clinical Diagnostics, Incorporated
International Classes:
C12N15/09; C12Q1/686; C12Q1/68; C12Q1/70
Domestic Patent References:
| JP5292968A | ||||
| JP7163370A |
Other References:
Proc. Natl. Acad. Sci. USA, (1994.03.15), 91, [6], p.2216-2220
Proc. Natl. Acad. Sci. USA, (1994.6.7), 91, [12], p.5695-5699
Proc. Natl. Acad. Sci. USA, (1994.6.7), 91, [12], p.5695-5699
Attorney, Agent or Firm:
Takashi Ishida
Yoshio Yoshio
Toshio Toda
Masaya Nishiyama
Yoshio Yoshio
Toshio Toda
Masaya Nishiyama