オリゴヌクレオチドN3’→P5’ホスホルアミデート:合成および化合物;ハイブリダイゼーションおよびヌクレアーゼ耐性特性

Japanese Patent JP4383720

B2

PCT No. PCT/US95/03575 Sec. 371 Date Jan. 10, 1997 Sec. 102(e) Date Jan. 10, 1997 PCT Filed Mar. 20, 1995 PCT Pub. No. WO95/25814 PCT Pub. Date Sep. 28, 1995Modified oligonucleotides 3'-NHP(O)(O-)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.

WO/2011/142783	MODIFIED CLOSTRIDIAL TOXINS COMPRISING AN INTEGRATED PROTEASE CLEAVAGE SITE-BINDING DOMAIN
WO/2022/043531	7'-SUBSTITUTED 2'-O-4'-C-ETHYLENE-BRIDGED NUCLEIC ACID (ENA) MONOMERS AND USES THEREOF
WO/1989/006702	SELECTIVE INHIBITION OF GENE EXPRESSION BY PHOTOACTIVATABLE OLIGONUCLEOTIDES

Sergey M. Grinob
Ronald G. Schultz
Changer-Can

JP2002171743A

December 16, 2009

June 12, 2002

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GERON CORPORATION

C07H21/00; A61K31/70; C12Q1/02; A61K31/7042; A61K31/7052; A61K31/7076; A61K31/708; A61K31/7088; A61K31/7125; A61K48/00; A61P29/00; A61P31/04; A61P31/10; A61P31/12; A61P35/00; A61P35/02; A61P43/00; C07H19/06; C07H19/16; C07H19/173; C07H21/04; C12N15/09; C12Q1/68; G01N33/50; G01N33/566

Tetrahedron Letters,1993年,Vol.34, No.38,p.6017-6020
Alexei V.Azhayev et al.,Synthesis of phosphoramidate analogues of short oligoribonucleotides,Nucleic Acids Research Symposium Series,英国,IRL Press limited.,1981年,No.9 ,PP251-254
NUCLEIC ACIDS RESEARCH,1987年,Vol.15 No.4,P1699-1715
NUCLEIC ACIDS RESEARCH,1992年,Vol.20 No.13,P3403-3409

Hidesaku Yamamoto

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