Title:
オリゴヌクレオチドプローブ及びその利用
Document Type and Number:
Japanese Patent JP5709104
Kind Code:
B2
Abstract:
The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C 2 or C 3 alkylene chain, R2 represents an optionally substituted C 0-2 alkylene chain, and Z represents a direct bond or linker.)
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Inventors:
Hiroyuki Asanuma
Liang Xingguk
Kei Kashida
Yasuko Yoshida
Tomokazu Takase
Kosuke Niwa
Liang Xingguk
Kei Kashida
Yasuko Yoshida
Tomokazu Takase
Kosuke Niwa
Application Number:
JP2010519079A
Publication Date:
April 30, 2015
Filing Date:
June 30, 2009
Export Citation:
Assignee:
Nagoya University
International Classes:
C12M1/00; C12Q1/68; C12N15/09
Domestic Patent References:
| JPS62157570A | 1987-07-13 |
Foreign References:
| EP0601889A2 | 1994-06-15 |
Other References:
JPN6009043301; 樫田啓: '二重鎖形成を可視化するプローブＤＮＡの開発' 高分子学会予稿集 ５２巻，第５号, 20030508, １０４４頁，ＩＩＰｄ１６４, 社団法人高分子学会
JPN6015006113; Ｏｒｇ． Ｂｉｏｍｏｌ． Ｃｈｅｍ． Ｖｏｌ． ６, 200806, ｐｐ．２８９２-２８９９
JPN6015006113; Ｏｒｇ． Ｂｉｏｍｏｌ． Ｃｈｅｍ． Ｖｏｌ． ６, 200806, ｐｐ．２８９２-２８９９
Attorney, Agent or Firm:
Kaiyu International Patent Office