Title:
ワンステップ逆転写テンプレートスイッチＰＣＲ
Document Type and Number:
Japanese Patent JP6924496
Kind Code:
B2
Abstract:
The present invention provides a method for analyzing the variable region repertoire of the T-cell receptors (TCR) or the B-cell receptors (BCR) of a subject, said method including: (1) a step for providing a nucleic acid sample that is amplified from RNA obtained from the subject using one-step reverse transcription template-switching PCR and that includes the nucleic acid sequences of a plurality of types of T-cell receptors (TCR) or B-cell receptors (BCR); (2) a step for determining the nucleic acid sequences included in the nucleic acid sample; and (3) a step for calculating the frequencies of occurrence of individual genes or combinations thereof on the basis of the determined nucleic acid sequences and deriving the TCR or BCR repertoire of the subject.
Inventors:
Katsuyuki Shiroguchi
Application Number:
JP2018523697A
Publication Date:
August 25, 2021
Filing Date:
June 23, 2017
Export Citation:
Assignee:
RIKEN
International Classes:
C12Q1/686; C12N15/11
Domestic Patent References:
| JP2011521646A | ||||
| JP2000502905A | ||||
| JP2009538621A |
Other References:
Jeremy KAPTEYN, et al.,BMC Genomics,2010年,Vol.11:413,pp.1-9
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto