Title:
ＰＣＶ２免疫原性組成物及びそのような組成物を製造する方法
Document Type and Number:
Japanese Patent JP5726395
Kind Code:
B2
Abstract:
An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
Inventors:
Eye bearer mark
Nitzel Greg
Schaefer Merrill
Nitzel Greg
Schaefer Merrill
Application Number:
JP2007549685A
Publication Date:
May 27, 2015
Filing Date:
December 29, 2005
Export Citation:
Assignee:
Boehringer Ingelheim Vemedica INC.
International Classes:
C12P21/02; A61K38/00; A61K39/12; A61K39/39; A61P31/12; A61P31/20; C12N15/09
Domestic Patent References:
| JP2003522106A |
Foreign References:
| US6497883 |
Other References:
J. Vet. Sci.,2002年,vol.3 no.1,pp.19-23
Journal of General Virology,2000年,vol.81,pp.2281-2287
Vaccine,2003年,vol.21,pp.4565-4575
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,2002年,vol.9 no.1,pp.33-40
Journal of General Virology,2000年,vol.81,pp.1815-1824
Journal of General Virology,2000年,vol.81,pp.2281-2287
Vaccine,2003年,vol.21,pp.4565-4575
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,2002年,vol.9 no.1,pp.33-40
Journal of General Virology,2000年,vol.81,pp.1815-1824
Attorney, Agent or Firm:
Nozomi Watanabe
Haruko Sanwa
Haruko Sanwa