Title:
【発明の名称】ウイルス生産に有用なヒト胎児肺線維芽細胞二倍体細胞株およびこれを用いた水痘帯状疱疹ウイルスの製造方法
Document Type and Number:
Japanese Patent JP2944223
Kind Code:
B2
Abstract:
PCT No. PCT/KR97/00028 Sec. 371 Date Oct. 14, 1997 Sec. 102(e) Date Oct. 14, 1997 PCT Filed Feb. 15, 1997 PCT Pub. No. WO97/30147 PCT Pub. Date Aug. 21, 1997A human embryonic lung fibroblast diploid cell strain LBHEL(KCTC 0127BP) is highly susceptible to varicella zoster virus(VZV). A cell-free varicella zoster virus (VZV) is produced by (a) culturing the human embryonic lung fibroblast diploid cell strain LBHEL(KCTC 0127BP) of claim 1 in a culture vessel to give cultured LBHEL cells; (b) infecting the cultured LBHEL cells with VZV to give VZV-infected cells; (c) culturing and harvesting the VZV-infected cells; (d) disrupting the harvested cells to obtain a cell homogenate; and (e) centrifuging the cell homogenate to obtain a supernatant containing the cell-free VZV.
Inventors:
KIMU JON MIN
PAKU BOKU RYON
PAKU JU HON
PAKU BOKU RYON
PAKU JU HON
Application Number:
JP52921197A
Publication Date:
August 30, 1999
Filing Date:
February 15, 1997
Export Citation:
Assignee:
ERU JI KAGAKU KK
International Classes:
A61K39/12; A61K39/25; C12N5/071; C12N7/00; C12N7/02; (IPC1-7): C12N5/06; C12N7/02
Attorney, Agent or Firm:
Aoyama Ryo (2 outside people)