Title:
【発明の名称】高濃度糖混合液からの高純度オリゴ糖類の製造法
Document Type and Number:
Japanese Patent JP3095643
Kind Code:
B2
Abstract:
PURPOSE: To provide a method for producing a high purity oligosaccharides in a high yield, having dental caries resistant, hardly digestible and having lipids reducing properties by effecting an α-glucosidase to a highly concentrated mixed saccharide solution containing sucrose and oligosaccharides and then removing the produced monosaccharides. CONSTITUTION: This method for producing a high purity oligosaccharides in purifying the oligosaccharide from a highly concentrated mixed saccharide solution having >=30 Bx. containing sucrose and oligosaccharides (e.g. fructo- oligo saccharide) comprises adding an α-glucosidase produced by Saccharomyces pastorianus IFO751 to the mixed saccharide solution, reacting at 37 deg.C for 12hr to decompose the sucrose into monosaccharides, then deactivating the enzyme by boiling the mixed solution for 10min and removing monosaccharides from the mixed saccharide solution by a chromatographic method to obtain the high purity oligosaccharides having dental caries resistant and hardly digestable properties, a specific growth promoting effect on Lactobacillus bifidus in the intestine, and a reducing effect on in vivo lipids in a high yield.
Inventors:
Hitoshi Matsumoto
Koji Nishizawa
Takashi Kawakami
Masao Hirayama
Akira Adachi
Koji Nishizawa
Takashi Kawakami
Masao Hirayama
Akira Adachi
Application Number:
JP28308294A
Publication Date:
October 10, 2000
Filing Date:
November 17, 1994
Export Citation:
Assignee:
Meiji Seika Co., Ltd.
International Classes:
C12N9/26; C07H1/06; C07H3/04; C07H3/06; C12N9/24; C12P19/16; C12P19/18; C12S3/02; C12R1/85; (IPC1-7): C12P19/16; C07H3/04; C07H3/06; C12S3/02