Title:
【発明の名称】核酸の高感度検出方法
Document Type and Number:
Japanese Patent JP3360977
Kind Code:
B2
Abstract:
Method for the sensitive detection of a target nucleic acid by hybridization with a probe nucleic acid. The latter contains a part which can hybridize with the target nucleic acid and a nucleic acid-specific part which does not hybridize with the target nucleic acid. The method further comprises cleavage of the probe nucleic acid, hybridization of a cleavage product of the probe nucleic acid containing the part that does not hybridize with the target nucleic acid with a matrix nucleic acid containing a part that can be hybridized with the cleavage product and a part that cannot be hybridized with the probe nucleic acid. The method also comprises the determination of the hybrid consisting of the cleavage product and the matrix nucleic acid and a reagent kit suitable for this purpose.
Inventors:
Rudolf Zyble
Viola Rosemeyer
Viola Rosemeyer
Application Number:
JP17855095A
Publication Date:
January 07, 2003
Filing Date:
July 14, 1995
Export Citation:
Assignee:
Roche Diagnostic Gesell Shaft Mitt Beschlenktel Haftung
International Classes:
C07H21/00; C12N15/09; C12Q1/68; G01N33/58; (IPC1-7): C12Q1/68; C12N15/09; G01N33/58
Domestic Patent References:
| JP25864A |
Other References:
【文献】国際公開93/6240(WO,A1)
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)