Title:
PCR METHOD
Document Type and Number:
Japanese Patent JP2010162042
Kind Code:
A
Abstract:
To provide a method for balancing multiplexed PCR methods.
The method comprises two or more sequential temporal PCR stages used to effectively separate two or more PCR reactions in a single test tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during first and second amplification stages. Also provided are rapid RT-PCR methods that diagnose malignant esophageal adenocarcinoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.
COPYRIGHT: (C)2010,JPO&INPIT
Inventors:
GODFREY TONY E
LUKETICH JAMES D
RAJA SIVA
KELLY LORI A
FINKELSTEIN SYDNEY D
LUKETICH JAMES D
RAJA SIVA
KELLY LORI A
FINKELSTEIN SYDNEY D
Application Number:
JP2010060455A
Publication Date:
July 29, 2010
Filing Date:
March 17, 2010
Export Citation:
Assignee:
UNIV OF PITTSBURGH OF THE COMM
International Classes:
C12N15/09; G01N33/53; C12M1/00; C12N15/00; C12P19/34; C12Q1/02; C12Q1/68; C12Q1/6851; C12Q1/6886
Domestic Patent References:
| JP2000044823A | 2000-02-15 | |||
| JPH10332701A | 1998-12-18 | |||
| JPH06261757A | 1994-09-20 | |||
| JPH09508521A | 1997-09-02 |
Foreign References:
| WO2000032763A1 | 2000-06-08 | |||
| US6168948B1 | 2001-01-02 |
Attorney, Agent or Firm:
Shinjiro Ono
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Norihiro Fukasawa
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Norihiro Fukasawa
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