Title:
Pep-ELISA MEASUREMENT PEPTIDE
Document Type and Number:
Japanese Patent JP2015108597
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide an epitope peptide supporting stand peptide maintaining orientation of the epitope peptide when immobilizing the epitope peptide on a substrate, and realizing improvement in detection sensitivity and stable reproducibility, and to provide a Pep-ELISA method using the stand peptide.SOLUTION: Provided is a stand peptide that is a peptide for immobilizing a measurement target peptide aptamer on a substrate and that has an array of an array number 1 in an array table in a Pep-ELISA method. Further provided is a Pep-ELISA method including: a step of immobilizing the stand peptide on the substrate by a C terminal or an N terminal; a step of bonding the peptide aptamer with the immobilized stand peptide; a step of bonding a target molecule with the bonded peptide aptamer; a step of further bonding a target antibody for the target molecule with the target molecule; and a step of detecting a reaction produced by bonding of the antibody with the target molecule by fluorescence measurement or absorbance measurement.
Inventors:
NISHIGAKI KOICHI
KITAMURA KOICHIRO
YAMAMOTO KENJI
KITAMURA KOICHIRO
YAMAMOTO KENJI
Application Number:
JP2013252485A
Publication Date:
June 11, 2015
Filing Date:
December 05, 2013
Export Citation:
Assignee:
UNIV SAITAMA
JANUSYS KK
JANUSYS KK
International Classes:
G01N33/543; C07K7/08; G01N33/573
Domestic Patent References:
| JP2005220028A | 2005-08-18 | |||
| JP2009109453A | 2009-05-21 | |||
| JP2012211207A | 2012-11-01 | |||
| JP2011168505A | 2011-09-01 | |||
| JP2010107491A | 2010-05-13 |
Foreign References:
| US5482867A | 1996-01-09 |
Attorney, Agent or Firm:
Fujita Shibata
Shibata Goo
Shibata Goo