PURPOSE: To provide a new peptide containing an amino acid sequence such as L-leucine, L-glutamine, L-proline, etc., having hypotensive action and useful as a treating agent for essential hypertension, etc., as an angiotensinase inhibitor.
CONSTITUTION: Wheat gluten is added with distilled water, heat-treated at 121°C for 10min, added with 1M sodium citrate buffer solution (pH 2.0), adjusted to pH 2.0 with 1N-HC1, added with molsin (an acidic protease) and stirred at 30°C for 44hr to effect enzymatic reaction. The obtained hydrolyzate is heated at 100°C for 10min to deactivate the residual enzyme and the insoluble materials are removed by centrifugal separation to obtain a filtrate. The filtrate is condensed and then purified by successively subjecting to gel-filtration chromatography and reversed phase high-performance liquid chromatography to obtain the objective peptide expressed by formula (R1 is H or amino acid residue; R2 is hydroxyl or amino acid residue) and having an amino acid polymerization degree of 3-6.
YAMAKOSHI JUN
KINOSHITA EMIKO
KOGA TAKUO
NAKAJIMA YASUHIKO
KIKUCHI MAMORU
