Title:
ペプチドリガーゼ及びその使用
Document Type and Number:
Japanese Patent JP7075678
Kind Code:
B2
Abstract:
The present invention relates to a polypeptide that is capable of promoting the covalent conjugation of two peptide tags or linkers and in particular to a polypeptide comprising: a) an amino acid sequence as set forth in SEQ ID NO: 1; or b) an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 1, wherein said amino acid sequence comprises a glutamic acid at position 61 and one or more of the following: 1) proline at position 66; 2) proline at position 95; 3) glycine at position 96; and 4) valine at position 97, wherein the specified amino acid residues are at positions equivalent to the positions in SEQ ID NO: 1 and wherein said polypeptide is capable of promoting the formation of an isopeptide bond between the lysine residue at position 9 of SEQ ID NO: 2 and the asparagine residue at position 17 of SEQ ID NO: 3.
Inventors:
Haworth mark
Burdung Can M
Burdung Can M
Application Number:
JP2020504454A
Publication Date:
May 26, 2022
Filing Date:
April 10, 2018
Export Citation:
Assignee:
Oxford University Innovation Limited
International Classes:
C12N15/52; C07K7/08; C07K14/315; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N15/31; C12N15/63; C12P21/02
Domestic Patent References:
| JP2015509713A |
Foreign References:
| WO2016193746A1 | ||||
| WO2011098772A1 |
Other References:
Fierer, J. O. et al.,Proc. Natl. Acad. Sci. U. S. A.,2014年,VOl. 111,pp. E1176-E1181
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes