Title:
【発明の名称】化学発光ラベル及びビオチン結合リガンドによる抗体の2サイトイムノアッセイ
Document Type and Number:
Japanese Patent JP3194585
Kind Code:
B2
Abstract:
A method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed against the antibody to be detected bound to paramagnetic particles; and a chemiluminescent acridinium compound bound to avidin or streptavidin to form a solid phase complex; separating the solid phase from the liquid phase; and analysing the separated solid phase for the presence of chemiluminescent complex.
Inventors:
Johannes, Niels
Ibsen, Hans-Henritz
Ibsen, Hans-Henritz
Application Number:
JP51161794A
Publication Date:
July 30, 2001
Filing Date:
November 15, 1993
Export Citation:
Assignee:
Are L Car A/S
International Classes:
G01N33/50; G01N33/533; G01N33/543; G01N33/546; G01N33/53; G01N33/553; G01N33/58; G01N33/68; (IPC1-7): G01N33/543; G01N33/53; G01N33/553
Other References:
（１）ＣＬＩＮＩＣＡＬ ＡＮＤ ＥＸＰＥＲＩＭＥＮＴＡＬ ＡＬＬＥＲＧＹ，Ｖｏ．２２，（１９９２），Ｊ．Ｋｌｅｉｎｅ−Ｔｅｂｂｌｅ ｅｔ ａｌ．”Ｃｏｍｐａｒｉｓｏｎ ｂｅｔｗｅｅｎ ＭＡＧＩＣ ＬＩＴＥ −ａｎｄ ＣＡＰ−ｓｙｓｔｅｍ：ｔｗｏ ａｕｔｏｍａｔｅｄ ｓｐｅｃｉｆｉｃ ＩｇＥ ａｎｔｉｂｏｄｙ ａｓｓａｙｓ．”，ｐ．４７５−４８４
（２）アレルギー「酵素免疫測定法による総ＩｇＥおよび特定的ＩｇＥ抗体の測定」第３０巻，第１１号，（１９８１），Ｐ．１０５２−１０５９
（２）アレルギー「酵素免疫測定法による総ＩｇＥおよび特定的ＩｇＥ抗体の測定」第３０巻，第１１号，（１９８１），Ｐ．１０５２−１０５９
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)