Title:
光切替可能ポリペプチドおよびその使用
Document Type and Number:
Japanese Patent JP7221537
Kind Code:
B2
Abstract:
The present invention relates to a light-switchable polypeptide. In particular, the present invention relates to a polypeptide comprising a light-responsive element, wherein the configuration (i.e. the configurational state) of the light-responsive element can be switched between a trans and cis isomer by irradiating the polypeptide with (a) particular wavelength(s) of light, and wherein the switch of said configuration alters the conformation and binding activity of said polypeptide to a ligand (e.g. molecule of interest). Also, the present invention comprises using said light-switchable polypeptide for isolating and/or purifying a molecule of interest. The present invention further provides an affinity matrix, an affinity chromatography column, and an affinity chromatography apparatus comprising the light-switchable polypeptide of the invention.
Inventors:
Arne Skela
Andreas Reichert
Martin Downer
Andreas Reichert
Martin Downer
Application Number:
JP2019561841A
Publication Date:
February 14, 2023
Filing Date:
May 09, 2018
Export Citation:
Assignee:
Technische Universitat Munich
International Classes:
C07K14/36; B01J20/281; B01J20/285; C07K1/22; C12M1/00; G01N30/88; G01N33/531; G01N33/543
Domestic Patent References:
| JP2003310253A | ||||
| JP2008513040A | ||||
| JP2001346579A | ||||
| JP2010260032A | ||||
| JP2003329682A | ||||
| JP2009077660A |
Other References:
Hoppmann, C. et al.,"In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light",J. Am. Chem. Soc.,2015年,Vol. 137,pp. 11218-11221
Shimoboji, T. et al.,"Photoswitching of ligand association with a photoresponsive polymer-protein conjugate",Bioconjug. Chem.,2002年,Vol. 13,pp. 915-919
Shimoboji, T. et al.,"Photoswitching of ligand association with a photoresponsive polymer-protein conjugate",Bioconjug. Chem.,2002年,Vol. 13,pp. 915-919
Attorney, Agent or Firm:
Aoki Atsushi
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Kumi Kitamura
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Kumi Kitamura