Title:
IL-2発現に適したプラスミド
Document Type and Number:
Japanese Patent JP3710815
Kind Code:
B2
Abstract:
The present invention relates to plasmids suitable for IL-2 expression, particularly, human IL-2 expression, and related methods. The plasmid consists essentially of an expression facilitating sequence derived from the immediate-early promoter region of CMV, an expression facilitating sequence derived from the transcriptional termination/polyadenylation signal sequence of the BGH gene, a sequence coding for the eukaryotic expression of an IL-2, possessing a bioactivity of the complete IL-2, operably linked to both of said expression facilitating augments eukaryotic expression compared to a wild-type IL-2 leader peptide. Optionally, the plasmid also includes a non-mammalian origin of replication and a sequence operably encoding a selectable marker.
Inventors:
Hobart, Peter, M.
Margaris, Mycal
Parker, Susanne, Yi.
Hathibi, Shrine
Margaris, Mycal
Parker, Susanne, Yi.
Hathibi, Shrine
Application Number:
JP51887196A
Publication Date:
October 26, 2005
Filing Date:
November 28, 1995
Export Citation:
Assignee:
Baikal Incorporated
International Classes:
C12N15/09; A61K31/70; A61K35/76; A61K38/00; A61K38/20; A61K47/44; A61K48/00; A61P35/00; C07K14/55; C12N5/08; C12N15/26; C12N15/62; C12N15/85; C12P21/02; C12P21/04; (IPC1-7): C12N15/09; A61K35/76; A61K38/00; A61K48/00; A61P35/00; C07K14/55; C12P21/02
Domestic Patent References:
| JP62278994A |
Other References:
MONTGOMERY D. L. et al., Hetetologous and Homologous Protection Against Influenza A by DNA Vaccination:Optimization of DNA Ve, DNA AND CELL BIOLOGY, 1993年,VOl.12,No.9, 777-783
PARKER S. E. et al., DIRECT DELIVERY OF CYTOKINE GENES INTO TUMORS FOR CANCER IMMUNOTHERAPY, JOURNAL OF CELLULAR BIOCHEMISTRY SUPPLEMENT 18A, 米国,1994年 1月,244
PARKER S. E. et al., DIRECT DELIVERY OF CYTOKINE GENES INTO TUMORS FOR CANCER IMMUNOTHERAPY, JOURNAL OF CELLULAR BIOCHEMISTRY SUPPLEMENT 18A, 米国,1994年 1月,244
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama